Home

Description

Search Catalogues

Browse catalogues

Collections

Prices

Guidelines
CELL LINES
PLANT CELL LINES
PLANT VIRUSES
PLASMIDS
PHAGES
MICROORGANISMS
GENOMIC LIBRARIES
PROCEDURES
CATALOGUE

Search Web Site

Contacts

FAQ

Site Map

LABORATORY PROCEDURES FOR PLANT CELL LINES

REFERENCE NO.: PC/1998/3/1.6


TITLE: DESCRIPTION OF METHODS FOR STANDARD HPLC ANALYSIS


INTRODUCTION

The following conditions and methods are used for the standard HPLC analysis of methanolic extracts.

PROCEDURE

Equipment: The following equipment is used: solvent delivery system: 2 pumps contraMetric 3000, LDC Analytical, 1990; detector: SM4000, LDC Analytical, 1990; autosampler: Marathon, Spark Holland, 1990; Dynamic + Static HPLC Mixing Chamber, Spark Holland, 1990; pump control and data analysis system: Thermochrom Vers. IV, LDC Analytical, 1990.)

Solvents used: Solvent A: Water + 0.01 % phosphoric acid

Solvent B: Methanol + 0.01 % phosphoric acid

Column
for routine standard: 4 x 100 mm, Nucleosil C18, 7 mm
for analytical standard: 4 x 250 mm, Nucleosil C18, 7 mm

Flow rate: 1 ml/min

Detection: wavelength: 280 nm, range: 0.01

Gradients:

Method 1

0 min; 100% solvent A; 0% solvent B; 1ml/min
1 min; 100% solvent A; 0% solvent B; Injection
31 min; 0% solvent A; 100%solventB;
36 min; 0% solvent A; 100%solventB;
31 min; 100% solvent A; 0%solventB;
44 min; 100% solvent A; 0% solvent B;

Method 6 (Prewash)

0 min; 0% solvent A; 100% solvent B; 0ml/min
1 min; 0% solvent A; 100% solvent B; 1ml/min
15 min; 100% solvent A; 0% solvent B;

Method 7 (Postwash)

0 min; 100% solvent A; 0% solvent B; 1ml/min
10 min; 0% solvent A; 100%solventB;
14 min; 0% solvent A; 100% solvent B; 1ml/min
15 min; 0% solvent A; 100 %solvent B; 0 ml/min

 


Guidelines prepared for CABRI by DSMZ, 20 Jan. 1998
Page Layout by CERDIC
Copyright CABRI, 1998 

© The CABRI Consortium 1999 - 2023
This work cannot be reproduced in whole or in part without the express written permission of the CABRI consortium.
Site maintained by Paolo Romano. Last revised on February 2023.