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LABORATORY PROCEDURES FOR ANIMAL & HUMAN CELL LINES

Quality controls at ECACC

 

Identification of cell line		Mycoplasma testing
Reference Number	Procedure Title	Reference Number	Procedure Title
AHC/1998/3/3.1/2.1	Cell enumeration by vital dye exclusion	AHC/1998/3/3.2/2.1	Mycoplasma detection by Hoescht stain
AHC/1998/3/3.1/2.2	Isoenzyme analysis	AHC/1998/3/3.2/2.2	Mycoplasma detection by culture
AHC/1998/3/3.1/2.3	DNA fingerprinting	AHC/1998/3/3.2/1.5	Mycoplasma eradication

 

Bacteria and fungi testing		Virus testing
Reference Number	Procedure Title	Reference Number	Procedure Title
AHC/1998/3/3.3/2.1	Bacteria and Fungi detection by culture	AHC/1998/3/3.4/2.1	Isolation of cell line RNA for use in RT-PCR
AHC/1998/3/3.3/2.2	Bacteria and Fungi eradication	AHC/1998/3/3.4/2.2	BVDV detection by use of RT-PCR
		AHC/1998/3/3.4/2.3	BVDV detection by use of a DiG labelled probe following RT PCR
		AHC/1998/3/3.4/2.4	PCR analysis for HIV
		AHC/1998/3/3.4/2.5	PCR analysis for HTLV 1,2

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